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Evaluation of senescence-associated secretory phenotype (SASP) in conditioned medium (CM) of “young” (MmC−) and senescent (MmC+) MSCs: ( a ) representative image of the dot blot (Human XL Cytokine Antibody Array Kit) and protein production. Densitometric analysis of certain analytes in antibody arrays was quantified using ImageLab software 5.0 (Bio-Rad). ( b ) Multiplexed <t>fluorescent</t> bead-based <t>immunoassay</t> detection of cytokines in CM using Human Cytokine/Chemokine (48-plex) panel. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells.
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Evaluation of senescence-associated secretory phenotype (SASP) in conditioned medium (CM) of “young” (MmC−) and senescent (MmC+) MSCs: ( a ) representative image of the dot blot (Human XL Cytokine Antibody Array Kit) and protein production. Densitometric analysis of certain analytes in antibody arrays was quantified using ImageLab software 5.0 (Bio-Rad). ( b ) Multiplexed fluorescent bead-based immunoassay detection of cytokines in CM using Human Cytokine/Chemokine (48-plex) panel. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells.

Journal: Cells

Article Title: Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction

doi: 10.3390/cells13161345

Figure Lengend Snippet: Evaluation of senescence-associated secretory phenotype (SASP) in conditioned medium (CM) of “young” (MmC−) and senescent (MmC+) MSCs: ( a ) representative image of the dot blot (Human XL Cytokine Antibody Array Kit) and protein production. Densitometric analysis of certain analytes in antibody arrays was quantified using ImageLab software 5.0 (Bio-Rad). ( b ) Multiplexed fluorescent bead-based immunoassay detection of cytokines in CM using Human Cytokine/Chemokine (48-plex) panel. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells.

Article Snippet: Forty-eight cytokines were measured using multiplexed fluorescent bead-based immunoassay detection (MILLIPLEX ® MAP system, Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions with a Human Cytokine/Chemokine/Growth Factor Panel A—Immunology Multiplex Assay.

Techniques: Dot Blot, Ab Array, Software, Bead-based Assay

Cytokine concentration in conditioned medium of ECs and MSCs in monoculture, ECs cocultured with MSCs and ECs under conditioned medium from MSCs, evaluated using multiplexed fluorescent bead-based immunoassay. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells, ECs—endothelial cells, EC control—monoculture of ECs (untreated), MSC control—monoculture of MSCs (MmC− and MmC+), ECs + MSCs—coculture, ECs + CM of MSCs—CM-treated ECs.

Journal: Cells

Article Title: Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction

doi: 10.3390/cells13161345

Figure Lengend Snippet: Cytokine concentration in conditioned medium of ECs and MSCs in monoculture, ECs cocultured with MSCs and ECs under conditioned medium from MSCs, evaluated using multiplexed fluorescent bead-based immunoassay. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells, ECs—endothelial cells, EC control—monoculture of ECs (untreated), MSC control—monoculture of MSCs (MmC− and MmC+), ECs + MSCs—coculture, ECs + CM of MSCs—CM-treated ECs.

Article Snippet: Forty-eight cytokines were measured using multiplexed fluorescent bead-based immunoassay detection (MILLIPLEX ® MAP system, Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions with a Human Cytokine/Chemokine/Growth Factor Panel A—Immunology Multiplex Assay.

Techniques: Concentration Assay, Bead-based Assay, Control